Translational_Unit

Part:BBa_K3477008:Design

Designed by: Mathilda Johansson   Group: iGEM20_Chalmers-Gothenburg   (2020-10-20)


Gene coding for his-tagged MHETase enzyme including BBa_0034 RBS, Bba_J23106 promoter and Bba_0010 t


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1988
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 56
    Illegal PstI site found at 749
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal SpeI site found at 1989
    Illegal PstI site found at 749
    Illegal PstI site found at 2003
    Illegal NotI site found at 7
    Illegal NotI site found at 1996
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1989
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 56
    Illegal PstI site found at 749
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 56
    Illegal SpeI site found at 1989
    Illegal PstI site found at 749
    Illegal PstI site found at 2003
    Illegal NgoMIV site found at 849
    Illegal AgeI site found at 1201
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

The genetic sequence comes from Ideonella sakaiensis and was found on Uniprot (https://www.uniprot.org/uniprot/A0A0K8P8E7)


References